So far, courts across the country—all the way up to the U.S. Supreme Court—have sided with state governments on imposed vaccine requirements. The justices declined Friday to even hear a case challenging Maine’s vaccine mandate on religious grounds.

But now, the federal government is in the crosshairs of those who view President Joe Biden’s mandates as examples of government overreach.

Last week Washington, DC, District Judge Colleen Kollar-Kotelly issued a temporary restraining order and preliminary injunction prohibiting the firing of both civilian and active-duty military federal employees that already are required to be vaccinated while their religious exemption pleas are pending.

Michael Yoder, the attorney for the plaintiffs, told Fox News that the Biden administration has shown ‘an unprecedented, cavalier attitude toward the rule of law.’

The administration appeared to consider the case moot. In its filing Friday, it said, “Plaintiffs offer nothing beyond speculation to suggest that their religious exception requests will be denied and that they will be disciplined at all, much less on the first day that such discipline is theoretically possible,” according to Fox News.

In Florida, Gov. Ron DeSantis (R) has doubled down on his efforts to quelch the Department of Labor’s Office of Occupational Health and Safety Administration’s vaccine mandate, which is expected to be approved any day now. On Thursday, the governor filed a lawsuit against the Biden administration over the COVID-19 vaccine mandates for federal contractors.

The complaint, filed in federal district court in Tampa, calls the policy a “radical intrusion on the personal autonomy of American workers,” and seeks a preliminary nationwide injunction to block it from taking effect, The Hill reported. 

“It’s important for us to take a stand,” DeSantis said at a press conference. “Tossing people aside is just not something we can tolerate here in the state of Florida, so we are going to do everything we can.”

The lawsuit is separate from the governor’s response to OSHA’s expected emergency temporary standard, over which he said he stands ready for the state to sever its ties with the federal agency.

Jordan Barab, former OSHA deputy assistant secretary and former senior labor policy adviser to the House Committee on Education and Labor, said Thursday that individual states do not have the authority to opt out of the mandate.

“We are one nation with one workplace safety and health agency that’s positioned throughout the country, in all 50 states. Governors and other state officials have no authority to ignore federal laws or regulations they don’t like,” Barab said at a webinar hosted by the National Council for Occupational Safety and Health.

Iowa lawmakers are taking a different tack. Rather than fighting the inevitable OSHA mandate, the legislature passed a bill Thursday that would allow workers to collect unemployment benefits if they are fired for refusing to get vaccinated against COVID-19. 

“I believe we have found a meaningful solution to protect Iowans and Iowa businesses from the Biden administration’s extreme government overreach,” state House Speaker Pat Grassley (R-New Hartford) said in a statement, according to the Des Moines Register.